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Surface_Activated_Dynabeads

Magnetic Separation Technology

Surface-activated

Dynabeads?

A wide product portfolio for

flexible biomagnetic separations

Magnetic Separation Technology

The attraction is simply magnetisk *

Select from the large portfolio of surface-activated Dynabeads ?

Monosized beads for rapid and efficient separations →All-in-one-tube protocols, easy handling, and no sample loss →Manual or automated handling with superior reproducibility → A variety of ways to bind your ligand means flexibility for

→your research

Dynabeads? magnetic separation technology

The monodisperse and uniform Dynabeads? provide optimal acces-sibility and highly reproducible reaction kinetics, ensuring rapid and efficient binding of your target molecules under conditions causing minimal stress. Chemical agglutination and nonspecific binding are negligible with Dynabeads? compared to irregularly shaped magnetic particles (Figure 1).

The Dynabeads? disperse easily and are handled like a liquid. They exhibit no bead-to-bead magnetic attraction. Due to their superparamagnetic properties, they migrate to the magnet only when placed in a magnetic field. When the magnetic field is

* Magnetisk is the Norwegian word for magnetic. Did you know that Dynabeads? magnetic separation technology was pioneered in the 1980s by the Norwegian company Dynal,

now part of Invitrogen? To learn more, check out https://www.sodocs.net/doc/1c15634490.html,/dynal

Figure 1—Dynabeads? reduce variability in your research. A. The uniform, mono-disperse superparamagnetic Dynabeads? are manufactured with highly controllable product qualities and to a unique level of reproducibility within and between batches. B–D. Magnetic particles from alternative suppliers.

Figure 2—Dynabeads? allow for application flexibility. The Dynabeads? magnetic separation technology utilizes the gentle affinity interactions between bead-bound ligands and their specific targets. Protocols take place in a single tube, with just a few handling steps. Magnetic separation allows easy washing and concentration of your target material.

removed, the Dynabeads? immediately lose all their magnetic remanence and are easily resuspended.

Specific chemical groups facilitate binding of almost any ligand to the surface of the Dynabeads? for easy, convenient, and reliable isolation of your target (Figure 2).Surface-activated Dynabeads ?

Ligand-coupled Dynabeads ?

Magnetic Dynabeads ? with bound target

Add sample Isolated, bead-bound targets are easily washed and concentrated in a nal volume of your choice

Y Y

The Dynabeads? product portfolio

To meet the specific requirements of different applications, various sur-face-activated Dynabeads? are available in a range of different bead sizes and surface chemistries (see Table 1 and Ordering information). Different ligands (antibodies, proteins, etc.) require different bead surface proper-ties and immobilization chemistries. Some considerations include: Hydrophobic or hydrophilic:

→ Hydrophilic Dynabeads? allow gentle adsorption and coupling of ligands, keeping the functional activity of enzymes and labile proteins intact after immobilization.

Hydrophobic Dynabeads? are optimal for coupling of antibodies

for affinity purification of proteins or organelles.

Bead size:

→ For capture and handling of proteins, nucleic acids, or similar biomolecules, use the 1 μm Dynabeads? or the 2.8 μm

Dynabeads?. The larger 4.5 μm Dynabeads? are well suited to

isolate larger entities such as organelles or cells. Due to their size

and high iron content, they exert a strong pull to the magnet,

even in viscous samples, yet still retain gentle handling of the

bound target.

Coupling considerations:

→ First consider the nature of the ligand (see Table 2 for details). How labile is the ligand? What

active groups are available for coupling? The orientation of the

active site of the ligand should also be taken into consideration.

Hydrophobic beads facilitate interactions between the beads and the hydrophobic parts of a protein, while the hydrophilic beads are better suited when an interaction with the hydrophilic parts of the protein is desired.

Manual or automated handling:

→ The 1 μm Dynabeads? are ideal for isolation of smaller entities such as proteins and phages. These beads are tailor-made for use in automated protocols where high throughput is crucial. They have a large surface area and high

capacity, efficient magnetic pull, and excellent properties for

automation. Suitable protocols have been developed for several

commercially available robotic workstations.Table 1—Product guide. The large portfolio of surface-activated Dynabeads? a Surface-activated Dynabeads?:Dynabeads? M-270

Epoxy

Bead characteristics:

Hydrophilic bead

Surface epoxy groups

Bead size: 2.8 μm diameter

Binding properties:

Typically binds 5–10 μg IgG

per mg beads

Direct covalent binding to

primary amino and sulfhy-

dryl groups in proteins and

peptides

No further surface activation

required

Binding overnight at neutral

pH, high salt and a wide

temperature range

Active chemical functionality:100–200 μmol/g beads

Main benefits:

The functionality of your

enzyme is maintained after

isolation

Extremely low nonspecific

binding of proteins, dyes, etc.

is observed with these pH

neutral beads, reduced need

for blocking agents

Good orientation of coupled

C C

allows for application flexibility.

Dynabeads? M-280 Tosylactivated Dynabeads? MyOne?

Tosylactivated

Dynabeads? M-500

Subcellular

Dynabeads? M-270

Carboxylic Acid

Dynabeads? MyOne?

Carboxylic Acid

Dynabeads? M-270

Amine

Hydrophobic bead ?

Surface tosyl groups ?

Hydrophobic bead

Surface tosyl groups

Slightly hydrophobic

bead

Surface tosyl groups

Hydrophilic bead

Surface carboxylic acid

groups

Hydrophilic bead

Surface carboxylic acid

groups

Hydrophilic bead

Surface amino groups

2.8 μm diameter

? 1 μm diameter

? 4.5 μm diameter

? 2.8 μm diameter

? 1 μm diameter

? 2.8 μm diameter

Typically binds

10–15 μg IgG per mg beads

Direct covalent

binding to primary amino and sulfhydryl groups in proteins and peptides

No further surface ?

activation required Binding overnight at ?

neutral to high pH and 37°C

Typically binds

15–20 μg IgG per mg

beads

Direct covalent

binding to primary

amino and sulfhydryl

groups in proteins and

peptides

No further surface

activation required

Binding overnight at

neutral to high pH and

37°C

Typically binds 2–3 μg

IgG per mg beads

Direct covalent

binding to primary

amino and sulfhydryl

groups in proteins and

peptides

No further surface

activation required

Binding overnight at

neutral to high pH and

37°C

Typically binds 5–10 μg

IgG per mg beads

Covalent amide bond

formation with primary

amino groups in pro-

teins and peptides

Activation through car-

bodiimide is required

Immediate peptide

bond formation at

pH 5–6 and room

temperature

Typically binds 10–15

μg IgG per mg beads

Covalent amide bond

formation with primary

amino groups in pro-

teins and peptides

Activation through car-

bodiimide is required

Immediate peptide

bond formation at

pH 5–6 and room

temperature

Typically binds 5 μg

IgG per mg beads

Direct covalent bind-

ing through reductive

amination of aldehydes

No further surface

activation required

Rapid binding (less

than 1 hour) at neutral

to high pH and room

temperature

100–200 μmol/g beads

? 40–60 μmol/g beads

? Not determined

? 200–250 μmol/g beads

? 400–800 μmol/g beads

? 100–200 mmol/g

High capacity for

isolating proteins by using an immobilized antibody

Optimal orientation of ?

coupled antibodies, typically bound via the more hydrophobic Fab region

High capacity for

isolating proteins by

using an immobilized

antibody

Optimal orientation of

coupled antibodies,

typically bound via the

more hydrophobic Fab

region

Five layers of coating

ensures a supersmooth

bead surface, perfect

for EM-imaging of

captured organelles

Optimal orientation of

coupled antibodies,

typically bound via the

more hydrophobic Fab

100% covalent binding

Rapid binding

chemistry

Low nonspecific bind-

ing of nucleic acids

100% covalent binding

Rapid binding

chemistry

Low nonspecific bind-

ing of nucleic acids

Rapid immobilization

of carbohydrates,

glycoproteins, and

glycolipids (e.g.,

lipopolysaccharides)

Easy introduction of

further alternative

surface chemistries O

S CH

COOH3

Table 2—Select the optimal bead for your specific application.

Ligand

Target

Dynabeads? Epoxy

Dynabeads? Tosylactivated

Dynabeads? Subcellular

Dynabeads? Carboxylic Acid

Dynabeads? Amine

Antibody

Low MW antigen 1 or peptide ? ?? ? ?? ?? ?Protein or antibody ? ?? ? ?? ??Protein complex

? ? ?? ???Organelles ? ?2? ?? ? ?

Phage 1? ?? ? ?? ?? ?Virus ? ?? ? ?? ?

? ?

Bacteria ? ? ?Cells 3

? ? ?? ? ?Antibody fragment Phage 1 or antibody ? ?? ?? ? ?? ? ?Protein Phage 1 or carbohydrate ? ? ?? ?? ?? ?Nucleic acid ??? ? ??Peptide Phage 1 or antibody ? ?? ?? ? ?? ? ?Carbohydrate Antibody ??? ? ?Low MV antigen Antibody ? ?

? ?? ?? ?Nucleic acids, oligonucleotide, aptamer, PNA

Nucleic acid binding proteins 1?

? ?? 4DNA 1, RNA 1 or PCR amplicons 1

? ? ?

? 4Enzyme

Substrate or

target for enzyme degradation

? ? ?? ???Organic chemistry derivatiza-tion, including introduction of new functional groups

? ?

? ? ?

??? best product choice for listed ligand and target,?? alternative choice for listed ligand and target, ? can be used for listed ligand and target. 1

Dynabeads? pre-coupled with streptavidin may be the best product choice. 2

Dynabeads? M-450 Epoxy. 3

Dynabeads? M-450 are recommended for isolation, stimulation, and expansion of cells.4

Dynabeads? Amine is modified with an appropriate bifunctional crosslinker prior to immobilization of oligonucleotides.

large protein complexes and the native state of proteins. No col-umns or centrifugations are needed.

Dynabeads? bring reproducibility and robustness to your research. The attraction is simply magnetisk .

https://www.sodocs.net/doc/1c15634490.html,

Ordering information

Products

Concentration

Quantity

Cat. no.

Dynabeads? M-270 Epoxy

Hydrophilic 2.8 μm beads with epoxy groups 60 mg 300 mg Freeze-dried 143-01143-02D Dynabeads? M-280 Tosylactivated

Hydrophobic 2.8 μm beads with tosyl groups 30 mg/ml 30 mg/ml 2 ml 10 ml 142-03142-04Dynabeads? MyOne? Tosylactivated

Hydrophobic 1 μm beads with tosyl groups 100 mg/ml

2 ml 10 ml 100 ml 655-01655-02655-03Dynabeads? M-500 Subcellular

Slightly hydrophobic 4.5 μm beads with tosyl groups 4 x 108 beads/ml 2 ml 150-01Dynabeads? M-270 Carboxylic Acid

Hydrophilic 2.8 μm beads with carboxylic acid groups 2 x 109 beads/ml 2 ml 10 ml 143-05D 143-06D Dynabeads? MyOne? Carboxylic Acid

Hydrophilic 1 μm beads with carboxylic acid groups 10 mg/ml

2 ml 10 ml 100 ml 650-11650-12650-13Dynabeads? -270 Amine

Hydrophilic 2.8 μm beads with amino groups

30 mg/ml

2 ml 10 ml

143-07D 143-08D

Surface_Activated_Dynabeads

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