pcDNA3.1-v5-his-topo_map

CMV promoter: bases 209-863

T7 promoter/priming site: bases 863-882Multiple cloning site: bases 902-1019TOPO ? Cloning site: 953-954V5 epitope: bases 1020-1061

Polyhistidine tag: bases 1071-1088

BGH reverse priming site: bases 1111-1128BGH polyadenylation signal: bases 1110-1324f1 origin of replication: bases 1387-1800SV40 promoter and origin: bases 1865-2190Neomycin resistance gene: bases 2226-3020SV40 polyadenylation signal: bases 3039-3277pUC origin: bases 3709-4382

Ampicillin resistance gene: bases 4527-5387

A