《myc基因的生物信息学分析》

Sh生命《myc基因的生物信息学分析》

海洋生命学院

0 《myc基因的生物信息学分析》

2015/7/3

课程：《生物信息学》

教师：张建业

时间：周三56节

教室：[实验中心111]

学生：

班级：2012级生物化学与分子生物学

学号：12050032003

The gene of myc in Rabbit

基因序列是从网站（https://www.sodocs.net/doc/fc17453505.html,/）上，依据以下步骤获得：

1）选择”Gene”和输入”myc”, click “Search”；

2）找到”rabbit”, 点击进入下一页面；

3）点击“FASTA”，获取核酸序列；

4）得到myc基因序列；

5）复制全序列，保存到记事本中即可，保存格式如下；

>Oryctolagus cuniculus (rabbit) [NC 013671.1] GGTGCGGGGGAAAGAGGTGTCTTTTTTTTTTTTTAATTATTATTTCTTTTCTCTTGGGTCCCCCTCTTCC TGGCCCCTTTGGGCAGGGAGTGGAGAGGGAGGCGGTGGAGCGCGCTGAAAGGGGAGTGGTCGGGGCTT GGCGGTGCGCGCTGCGCGGCGCTCCGGGTTTCGGGCTCCAGCGAGACCCTAACTCAAGGCTGCCCCCCCT TTGTGTGCCCCGCAGCAGGCTACGCGACGATGCCCCTCAACGTCAGCTTCGCCACCAACAGGAACTATGAC CTGGACTACGACTCGGTGCAGCCCTATTTCTACTGCGATGAGGAGGAGAACTTCTACCAGCAGCAGCAGCA GAGCGAGCTGCAGCCGCCGGCGCCCAGCGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCCCGCCC CTGTCCCCGAGCCGCCGCTCCGGCCTCTGCTCGCCCTCCTACGTTGCCGTCGCGTCCTTCTCCCCCAGGG GAGACGACGGCGGCGGCGGCGGCAGCTTCTCCACGGCTGACCAGCTGGAGATGGTCACCGAGCTGCTGG GCGGTGACATGGTGAACCAGAGCTTCATCTGCGACCCGGACGACGAGACCTTCATCAAGAACATCATCATC CAGGACTGCATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCGGAGAAGCTGGCCTCCTACCAGG CTGCGCGCAAGGACAGCGGCAGCCCGAGCCCCGCCCGCGGCCACGGCGGCTGCTCCACCTCCAGCCTGTA CCTGCAGGACCTGAGCGCCGCCGCCTCCGAATGCATCGACCCCTCGGTGGTCTTCCCCTACCCACTGCAC GACAGCAGCTCGCCCAAGCCCTGCGCCTCGCCCGACTCCAGCGCCTTCTCCCCGTCCTCGGACTCTCTGC TCTCCTCCAACGAGTCCTCTCCGCGGGCCAGCCCTGAGCCCCTGGTGCTCCATGAAGAGACACCGCCCAC CACCAGCAGCGACTCTGGTAAGCCTGAGGCCACTCCAGGCCGATCCAGGAGGGGGCGGGGTGGATGGAC GCCTGTCTGTTCTCACTGGCTGCTGATCAGTGGGGCACTTGAGCCCATTCTGCGTGCGTCTGTGCCGGTAT TTGGAAGGAGAAATGGCGGAGCTGGGACGGTGGGGCGCTCATCAACCCCGGAACCCTGGACTTTTTTAGA GTACCCTTGGCGCTTAGACCGTCCCAGTTTGCCATCCCCCCCCTTCTCTCCCACCCCTCAGGAATTTCAT TTGGGTTTTTAAATCTCCTGGCTTATCCATCCACTCCCTCTTACCTCTTAAGCATTTTAATTGCCTGGGG GGTGGGGGTGGGGGGGGCGTGTGAATGAGGATAAGAAAGGATTGATCTCCAGGAGTGAATGAATTGCCT GCCTCCCTTCCTTAACTTCTGAGAAGTGGCTGAAATTGAGTGGACTCAAGGCAGCTACAAGAATGAGGGGA GGGAGGCCAGCCTGCTGCCTGAGGGCTGCAGCTAGTGAAAGTGCCTATTCTGAACACTTAGAAGCAAAGC CTTGCCAAAACTAGACTTTTTTGCTCTTTCGCCCATCCCCCAGGACTTGTTGGCAAAGTTGTAAGATTTT TTTTTGCACTTCCAGTAAAATAGGGAGTTGCTAATATAGCACCTGAAGGTTCTTGGTAAAGTCCCTTAAA AATAGGAGGTGCTTGGGAACATGCTTGACTCTGGGTGTGTCCAAAGCCTCATTAAGTCTTAGGTAAGAAT

TGGCATCAATGTGATGTCCTGGCAAATTATAATTTTCTTGTCTGCACCACCACCCCAGCTGTCAATCTCC TCCCATTTAAATCTCACTTTGTAGGGGAGAAGAAACAGTGAGGCGTGTGCTTCTTTGGGGGTGCCGTTGT AACGTCCCAGTGCCTCCTGTTTGCATAGCATTCATTAAGCAAACAACGCAGTCATTGATTGTTGGGAAGC ATTGCTAACCGGGTGATTTCTGTTTCTTTTCTTAAAGAGGAAGAACAAGAGGATGAGGAAGAAATCGATG TCGTTTCTGTGGAAAAGAGGCAGCCCTCCACCAAAAGGTCAGGGTCACCATCTGCAGGGGGCCACAGCAA ACCTCCACACAGCCCGCTGGTCCTCAAGAGGTGCCACGTCTCCACCCATCAGCACAATTACGCCGCGCCC CCATCCACCAGGAAGGACTACCCAGCTGCCAAGCGGGCCAAGTTGGACAGTGGCCGAGTCCTGAAGCAGA TCAGCAACAATCGCAGATGTGCCAGCCCCAGGTCCTCAGACACCGAGGAGAACGACAAGAGGCGGACACA CAACGTCTTGGAACGCCAGAGGAGAAATGAGCTGAAAAGGAGCTTTTTTGCCCTGCGTGACCAGATCCCA GAGTTGGAAAACAATGAAAAGGCCCCCAAGGTAGTTATCCTTAAAAAAGCCACGGCGTACATCCTGGCCG TCCAAGCAGAGGAGCAAAAGCTCATTTCAGAGAAGGACTTGTTGCGGAAACGGCGAGAACAGTTGAAAC ACAAACTTGAACAGCTACGGAACTCTTGTGCATAAGTTGACCTATTGGAGGGAGGAACTGGACTGGCTCAT GAATTCTCATTTGTTACTAAGGGAAATTAAGGAAACAGGTTCCTTCTGACAGAACTGCAGCAATCCATTA CGTATGAACTTATTTCACATGCATGGTCAAGTGCAACCTCACAACCTTGGCTGGGTCTTGGGACTGTAAG GTTTAGCCATAAAGTAAACTGCCTCAAATGGGACTTTGGGCATAAAATAACTTTTTTTATGCTTGCCATC TTTTTTTTGTTTGTTTCTTTTAACAGATTTGTATTTAAGAATTGTTTTTAAAAAATTCTAAAGTTTACCC AACTTTCCTGTGTAAATATGGCCATTAAATGTAAATAACTTTAATAAAACGTTTATAGCAGTTATACAAG ATTTCAAGCCATGTATTATAAACCATAATTTTTTATTTAAGTACATTTTCCTTTTTAAAGTTGATTTTTT TCTATTGTTTTTAGAAAAAATAAAATACCTGGCAAATATATCATTGA

6）寻找到CDS序列；

>lcl|AF519451.1_cds_AAN03876.1_1 [protein=c-myc proto-oncogene] [partial=5',3']

[protein_id=AAN03876.1] [location=<1..>463] GGGTCACCATCTGCAGGGGGCCACAGCAAACCTCCACACAGCCCGCTGGTCCTCAAGAGGTGCCACGTCT CCACCCATCAGCACAATTACGCCGCGCCCCCATCCACCAGGAAGGACTACCCAGCTGCCAAGCGGGCCAA GTTGGACAGTGGCCGAGTCCTGAAGCAGATCAGCAACAATCGCAGATGTGCCAGCCCCAGGTCCTCAGAC ACCGAGGAGAACGACAAGAGGCGGACACACAACGTCTTGGAACGCCAGAGGAGAAATGAGCTGAAAAG GAGCTTTTTTGCCCTGCGTGACCAGATCCCAGAGTTGGAAAACAATGAAAAGGCCCCCAAGGTAGTTATCC TTAAAAAAGCCACGGCGTACATCCTGGCCGTCCAAGCAGAGGAGCAAAAGCTCATTTCAGAGAAGGACTT GTTGCGGAAACGGCGAGAACAGTTGAAACACAAACTTGAACAGC

7）将上述CDS序列的myc.txt格式直接改成myc.pro，然后用软件EditSeq打开，再将CDS 序列转化为氨基酸序列；

>lcl|AF519451.1_cds_AAN03876.1_1 [protein=c-myc proto-oncogene] [partial=5',3']

[protein_id=AAN03876.1] [location=<1..>463] GSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQISNNRRCASPRSSDTEEN DKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILAVQAEEQKLISEKDLLRKRREQLKHK LEQ

1 myc基因功能

1）my c基因既是一种可易位基因, 又是一种受多种物质调节的基因，具有促进细胞分

裂并获永生化功能。myc被认为是一种序列特异性转录因子而调节细胞的生长、分化及细胞程序化死亡。Myc 蛋白过度表达可以使细胞永生化，降低它们对生长因子需求，促进细胞循环进展，Myc 蛋白或许也促进染色体组不稳定因而导致恶性肿瘤。Myc 蛋白亦可作为一种独立的重要的细胞周期调节因子而参与恶性肿瘤的发生发展。

2）myc基因能够调节与细胞周期变化、细胞的生长代谢、基因的不稳定性、刺激血

管生成、细胞恶性转化、分化及凋亡的相关基因，如BCL-2。myc基因的异常表达可阻止细胞分化，引起细胞转化，活化的MYC 可以作为原癌基因参与肿瘤的形成及发展。在DLBCL 中，MYC 常与多与其他基因损伤及细胞遗传学异常相伴，如BCL-2 重排。

3）myc基因在肿瘤中被激活的方式有多种：①外源序列的插入激活。ALV病毒整合到B 淋巴细胞myc基因位点附近，随之插入病毒长末端重复序列（包含病毒启动子和增强子）使myc基因处在病毒启动子下而被活化。②染色体重排。这是myc基因激活的主要机制之一，在许多Burkitt”s淋巴瘤中发现有染色体特异性重排，其中有3处染色体移位，移位后的位点为编码免疫球蛋白基因所在，其表达相当活跃，故myc基因的转录因此增强。③基因扩增。癌基因可通过一定的机制在原来的染色体上复制出许多拷贝，为转录提供超量的模板，随着转录水平的增加，癌基因产物剧增，从而增加了转化蛋白的剂量，促使细胞恶性转化。myc基因扩增在肿瘤中是一种常见现象，可见于多种恶性肿瘤如鼻咽癌、乳腺癌、宫颈癌等。④点突变，较为少见。⑤DNA低甲基化：DNA甲基化是基因转录的重要调控方法，它的状态维持与基因转录活性密切相关。DNA转录活跃的区域通常甲基化程度较低，肿瘤组织DNA具有普遍的低甲基化特点，这种现象在癌前病变就出现,并随着肿瘤进展，低甲基化程度呈进行性下降。

2 Primer

2.1 引物设计原则

（1）引物长度：18~30bp；GC含量：一般在40%~60%之间；

（2）退火温度：一般情况下，一对引物的退火温度大致相等，可以设置一样温度；

（3）避免扩增模板的二级结构域：有关软件可以预测目的片段的稳定二级结构域，有助于选择模板。实验表明，若待扩区域的自由能（△G）小于58.6lkJ/mol，扩增往往不能成功。

（4）当被扩增的靶DNA序列较大，引物可能与靶DNA的多个地方结合，造成结果中有多个条带出现。

（5）引物3’端是延伸开始的地方。

①3’端不应超过3个连续的G或C，因这样会使引物在G+C富集序列区被错误引发。

②3′端也不能有形成任何二级结构可能，除在特殊的PCR（AS-PCR）反应中，引物3′

端不能发生错配。

③若扩增编码区域，引物3′端不要终止于密码子的第3位，因密码子的第3位易发

生简并，会影响扩增特异性与效率。

（6）引物自身不应存在互补序列,引物自身连续互补碱基不能大于3bp；两引物之间不应存在互补性，一对引物间不应多于4个连续碱基的同源性或互补性。

2.2 Design PCR Primers for DNA

将rabbit’s myc.txt（ORF）直接改成rabbit’s myc.seq；

（1）打开软件DNAMAN 8，然后打开”File”，选择”Open”，选择rabbit’s myc.seq；

（2）然后打开”Primer”，选择” Design PCR Primers for DNA”，出现设计引物的界面；

（3）设置参数，如下图所示；

（4）引物结果；

选择如下引物：

5’ primer：CATCCACCAGGAAGGACTAC；

3’ primer：TTTCAACTGTTCTCGCCG；

相关参数见上图；

2.3 Restriction Analysis

（1）点击”Restriction”，然后选择”Restriction Analysis”，然后进行参数设置，点击进入下一步；

（2）选择所有酶，进行匹配；

（3）酶切分析结果如下；

①Restriction analysis on lcl|AF519451.1_cds_AAN03876.1_1

Methylation: dam-Yes dcm-No

Screened with 117 enzymes, 5 sites found

BglI GCCNNNN/NGGC 1: 133

BstEII G/GTNACC 1: 2

Eco57I CTGAAGNNNNNNNNNNNNNNNN/ 1: 181

PstI CTGCA/G 1: 15

PvuII CAG/CTG 1: 124

List by Site Order

2 BstEII 124 PvuII 13

3 BglI 181 Eco57I 15 PstI

Non Cut Enzymes

AatII Acc65I AccIII AclI AflII AgeI

AhaIII Alw44I AlwNI ApaBI ApaI ApaLI

AscI Asp718I AsuII AvrII BalI BamHI

BbeI BbvII BclI BglII Bpu1102I Bsc91I

BsiI BsmI Bsp1407I BspHI BspMI BspMII

BssHII BstD102I BstXI Bsu36I ClaI Csp45I

CspI CvnI DraI DraIII DrdI EagI

Eam1105I Ecl136II Eco31I Eco47III Eco52I Eco56I Eco72I EcoICRI EcoNI EcoRI EcoRV EheI EspI FseI HindIII HpaI I-PpoI KpnI MfeI Mlu113I MluI MscI MstI MstII NaeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PinAI PmaCI PmeI PvuI RleAI SacI SacII SalI SapI SauI ScaI SciI SfiI SgrAI SmaI SnaBI SpeI SphI SplI SpoI SrfI SspI SstI SstII StuI SunI SwaI Tth111I VspI XbaI XcmI XhoI XmaI XmaIII XmnI XorII

Restriction sites on lcl|AF519451.1_cds_AAN03876.1_1

BstEII PstI

1 GGGTCACCATCTGCAGGGGGCCACAGCAAACCTCCACACAGCCCGCTGGTCCTCAAGAGG CCCAGTGGTAGACGTCCCCCGGTGTCGTTTGGAGGTGTGTCGGGCGACCAGGAGTTCTCC

61 TGCCACGTCTCCACCCATCAGCACAATTACGCCGCGCCCCCATCCACCAGGAAGGACTAC ACGGTGCAGAGGTGGGTAGTCGTGTTAATGCGGCGCGGGGGTAGGTGGTCCTTCCTGATG

PvuII BglI

121 CCAGCTGCCAAGCGGGCCAAGTTGGACAGTGGCCGAGTCCTGAAGCAGATCAGCAACAAT GGTCGACGGTTCGCCCGGTTCAACCTGTCACCGGCTCAGGACTTCGTCTAGTCGTTGTTA

Eco57I

181 CGCAGATGTGCCAGCCCCAGGTCCTCAGACACCGAGGAGAACGACAAGAGGCGGACACAC GCGTCTACACGGTCGGGGTCCAGGAGTCTGTGGCTCCTCTTGCTGTTCTCCGCCTGTGTG

241 AACGTCTTGGAACGCCAGAGGAGAAATGAGCTGAAAAGGAGCTTTTTTGCCCTGCGTGAC TTGCAGAACCTTGCGGTCTCCTCTTTACTCGACTTTTCCTCGAAAAAACGGGACGCACTG

301 CAGATCCCAGAGTTGGAAAACAATGAAAAGGCCCCCAAGGTAGTTATCCTTAAAAAAGCC GTCTAGGGTCTCAACCTTTTGTTACTTTTCCGGGGGTTCCATCAATAGGAATTTTTTCGG

361 ACGGCGTACATCCTGGCCGTCCAAGCAGAGGAGCAAAAGCTCATTTCAGAGAAGGACTTG TGCCGCATGTAGGACCGGCAGGTTCGTCTCCTCGTTTTCGAGTAAAGTCTCTTCCTGAAC

421 TTGCGGAAACGGCGAGAACAGTTGAAACACAAACTTGAACAGC

AACGCCTTTGCCGCTCTTGTCAACTTTGTGTTTGAACTTGTCG

②环状酶切图谱

3 Homologous Gene（BLAST）

3.1 FASTA之后，找到“Run BLAST”，点击进入下一页面；

3.2 选”blastn”，网页已经自动添加了文件：

3.3 选择database，一般选择”Nucleotide collection”，然后选择”Highly similar sequences(megablast)”；

3.4 click “BLAST”直接开始进行blast，后面的”Algorithm parameters”一般不用修改；

3.5 result：

3.6 选择其他同源序列：

根据“Query cover”的高低，选择了11种同源序列，结果如下：

> Felis catus (domestic cat)XM_011291443 CCGCACCCGCGAAACTTTGCCGGTTGCGGCGGGCGGACACTGTTCCCTGGAACTTACAACACCCGAGCAA

GAACGCGACTCTCCGGTCCGCCTATTTGGGGAAACACTTCTCCCCTACGCTGCCCGGGACCCGCTCCTCT GAAAGGGCGCTCCTCGCCGCTTTTCGGACGCTGGATTTCCTTCGGATAGTGGAAAACCCGCAGGCTGCCG CGATGCCCCTCAACGTCAGCTTCGCCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCCTATTT CTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCGCCGGCGCCCAG CGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCCCGCCGCTGTCCCCGAGCCGCCGCTCGGGGCTCT GCTCGCCCTCCTACGTCGCCTTCGCGTCCTTCTCCCCCCGGGGGGACGACGACGGCGGCGGCGGCAGCTT TTCCACGGCCGACCAGTTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAATCAGAGCTTCATC TGCGACCCGGACGACGAGACCTTCATCAAAAACATCATCATCCAGGACTGCATGTGGAGCGGCTTCTCGG CCGCCGCCAAGCTCGTCTCGGAGAAGCTGGCCTCCTACCAGGCTGCGCGCAAAGACAGCGGCAGCCCGAG CCCCGCCCGCGGGCCCGGAGGCTGCCCCACCTCCAGCTTGTACCTGCAGGACCTGACCGCCGCCGCCTCC GAGTGCATCGACCCCTCCGTGGTCTTCCCCTACCCGCTCAACGACAGCAGCTCGCCCAAGCCCTGCGCCT CCCCCGACTCCGCCGCCTTCTCCCCGTCCTCGGACTCTCTGCTCTCCTCGGCGGAGTCCTCCCCGCGGGC CAGCCCCGAGCCCCTGGCGCTCCACGAGGAGACACCGCCCACCACCAGCAGCGACTCTGAGGAAGAACAA GAGGAAGAAGAAGAAATTGATGTCGTTTCTGTGGAGAAAAGGCAGCCCCCTGCCAAAAGGTCGGAATCGG GGTCACCCTCTGCCGGAGGCCACAGCAAACCTCCTCACAGCCCGCTGGTCCTTAAGAGATGCCACGTGCC CACCCACCAGCACAATTACGCAGCGCCCCCCTCCACTAGGAAGGACTACCCAGCCGCCAAGAGGGCTAAG TTGGACAGTGGCAGGGTCCTGAAACAGATCAGCAACAACCGCAAATGCATCAGCCCCAGGTCTTCGGACA CGGAGGAGAACGACAAGAGGCGGACGCACAACGTCTTGGAACGCCAGAGGAGAAACGAGCTGAAACGG AGCTTTTTTGCCCTGCGCGACCAGATCCCAGAGTTGGAAAACAACGAAAAGGCCCCCAAGGTGGTGATCCT TAAAAAGGCCACCGCGTACATCCTGTCCGTCCAAGCAGGGGAGCAAAAGCTCATTTCGGAAAAGGACCTGT TGAGGAAGCGACGAGAACAGTTGAAACACAAACTTGAACAGCTAAGGAACTCTTGTGCATAAGTCCACCT ATTAGAGGGAGGAACTGGAGTTGCTCGTGAATTCTCACTTGTTACTAAGGGAAAGTAAGGAAAAAGCTTC CTTCTCACAGAACTGTAGCAACTCCTCATATCTGAACTTGTTTCAAATGCATGGTCAAGTGCAACCTCAC AACCTTGGCTGGGTCTTAGGATTGAAAGGTTTAGCCATAATGTAAACTGCCTCAAATGGAATTTTGGGCA TAAAAGAACATTTTTTTATGCTTGCCATCTTTTTGTTTGTTTGTTTTCCTTTAACAGATTTGTATTTAAG AATTGTTTTTAAAAAATGTGTCAAGTTTACCCCGTTTTCCTGTGTAAATATGGCCATTAAATGTAAATAA CTTTAATAAAACGTTTATAGCAGTTATACAAGAATTCAAACCATGTATTATAAACCATAATTTTTTTTAT TTAAGTACATTTTCCTTTTTAAAGTTGATTTTTTCTATTGTTTTTAGAAAAAATAAAATACGTGGCAAAT ATATAATTG

>Dasypus novemcinctus (nine-banded armadillo)XM_004458435.2 GTGTGGCCGTGGGGACGCGGTGTATTCGCGGCGATCTGAAGCTCAGCTGTTGTCACCGCGCTGATTTAAG CCCGGAGGACAAGTGTGCGGTTGGTCGAAACGAGCGCTGCGCCAGAGGACCCACAGAGAAAGGGAGAG GGGGGCTGAGCCCGAGCGAAAGAAAATGGTAGGCGCGGCAGGCCGGCGCGATGCCCCTCAACGTCAGCT TCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAAC TTCTACCACCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGCGCCCAGCGAGGATATCTGGAAGAAATTCG AGCTCCTGCCCACCCCTCCCCTGTCCCCGAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACAACTTTGCCT TCGCGTCCTTCTCCCCCGGGGGAGACGACGGCGGCGGCGGTGGCAGCTTTTCCACGGCCGACCAGCTGGA GATGGTGACCGAGCTGCTGGGGGGAGACATGGTGAACCAGAGCTTCATCTGCGACCCGGACGATGAGACC TTCATCAAAAACATCATCATCCAGGACTGCATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTGGTGTCGG AGAAGCTGGCCTCCTACCAGGCTGCGCGCAAAGACAGCAGCAGCCCAAGCCTCGCCCGGGGCCACAGCG GCTGCTCCACCTCCAGCCTGTACCTGCAGGACCTGAACGCCGCCGCCTCCGAGTGCATCGACCCCTCGGTG GTCTTCCCCTACCCGCTCAACGGCGGCGGTTCGCCCAAGCCCTGCCCCTCGCCGGATTCCACCGCCTTCT CTCCGTCCTCGGACTCTCTGCTCTCCTCCGTCGAGTCCTCCCCGCAGGCCAGCCCCGCACCCCTGGCCCT

CCACGAGGAGACGCCACCCACCACCAGCAGCGACTCAGAAGAAGAACAAGAGGATGAGGAAGAAATCGA TGTCGTGTCTGTGGAGAAGAGGCAGCCCCCTGCCAAGAGGTCAGATTCGGGGTCACCCTCCGCCGGAGGC CACAGCAAACCTCCTCACAGCCCCCTGGTCCTCAAGAGATGCCACGTCTCCACCCACCAACACAATTACGC AGCCCCGCCCTCCACCAGGAAGGACTTTCCCGCTGCCAAAAGGGCTAAGTTGGACAGTGGCCGAGTCCTG AAACAGATCAGCAACAACCGAAAGTGCGCCAGCCCCAGGTCTTCGGACACGGAGGAGAATGACAAGAGG CGGACGCACAACGTCCTAGAGCGCCAGAGGAGAAACGAGCTGAAGCGGAGCTTTTTTGCGCTGCGCGAC CAGATCCCCGAGTTGGAAAACAACGAAAAGGCCCCCAAGGTGGTTATCCTTAAAAAAGCCACAACGCACAT CCTATCCGTCCAAGCGGAGGAGCAAAAGCTCATGTCAGAAAAGGACTTGTTGCGAAAGCGGCGGGAACAG TTGAAACACAAACTCGAACAGCTACGGAACTCTGGGGCATAAGGTGGCCTATCGGAAGGGGGAACTGGAA TTGCTCATGAATTCTCACTTGTTACTAAGGGAAAGTAAGGAAAAGCGTTCCTTCTACCAGAACTGCAACCA TTCATTACCTATGAACTTACTTCAAACGCATGGTCAAGTGCAACCTCACAACCTTGGCTGGGTCTTGGGA CTGAAAGGTTTAGCCATAATGTAAACTGCCTCAAATGGGACTTTGGGCATAAAAACTTTTTTTATGCTTG CCGTCTTTTGTTTGTTTTTTTCTTTAACAGATTTGTATTTAAGAATTGTTTTTAAAAAAAGTCCTAAAAG TTTACCCAATTTTCCTGTGTAAATATGGCCATTAAATGTAAATAACTTTAATAAAACGTTTATAGCAGTT ATACAAGATTTGAAACCATGTATTGTATTATAAACCATAATTTTTTTTATTTAAGTACATTTTCCTTTTT AAAGTTGATTTTTTTTCTATTGTTTTTAGAAAAACTAAAAACCTGGCAAATATATAATT

>Camelus dromedarius (Arabian camel)XM_010988785.1 GAAGGGCAGGGCTATTCACGGGCTTGGCGCGAAAAAGAGAACAGAGGGGAGGGACGTGCCTGCTTGTAT AAAAGCCGGTTTTCTGGGCTTCAACTGACTCGCTGTAGTAATTCCAGCGAGAGGCAGAGGGAGCGAGCGG GCGGGCGCTTGGAGGGTGGAAGAGCCGAGCCAGCAGAGCGAGCAGAGTCGCGCTCCGGGGACCCGGG GAAGGGGAGATCCGGAGTGAAAGGGGGGCTTCGCCTCCGGCCCGGCCGCCCCCAACCCACTCTTCCCGTC GACCCCCGCCAGCAGTCCGCCACCCTCGCCACATCCACGAAACTTTGCCCACTGCGGCAGGCGGGCACTTT CCACTGGAACTTACAACACCGGAGCGACTCTCCCGACGCGGAGAGGCGATTCTGCCTATTTGGGGAGACG CTTTTCCCTGCCGCTGCCCACGACCCGCTCATCTGAAAGGCGCTCCACGCCGCCTTTTGGACGCTGGATTTC CTTCGGATAGTGGAAAACCCGCAGGCTGCCGCGATGCCCCTCAATGTCAGCTTCACCAACAGGAACTATGA CCTCGACTATGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAG CAGAGCGAGCTGCAGCCGCCGGCGCCCAGCGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCCCGC CCCTGTCCCCAAGCCGCCGCTCCGGGCTCTGCTCTCCCTCCTACGTTGCGGTCGCGTCCTTCTCCCCCAG GGGAGACGACGACGGCGGCGGCGGCTTTTCCACTGCTGACCAGTTGGAGATGGTGACCGAGCTGCTGGG AGGAGATATGGTGAACCAGAGCTTCATATGCGACCCGGACGACGAGACCTTCATCAAAAATATCATCATCC AGGACTGTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCCGAGAAGCTGGCCTCCTACCAGGC TGCGCGCAAAGACAGCAGCAGCCCGAGCCCCGCCCGTGGGCACGGCGGCTGCTCCACCTCCAGCCTGTAC CTGCAGGACCTAAGCGCCGCCGCCTCCGAATGCATCGACCCCTCCGTGGTCTTCCCCTACCCGCTCAACG ACAGCAGTTCGCCCAAGCCCTGCGCCTCCCCGGACTCCACCGCCTTCTCCCCGTCCTCGGACTCTCTGCT CTCCTCCGCCGAGTCGTCCCCGCGGGCCAGTCCCGAGCCCCTGGCGCTCCACGAGGAGACACCACCCACC ACCAGCAGCGACTCTGAGGAAGAACAAGAGGATGAGGAAGAAATTGATGTTGTTTCTGTAGAGAAGAGGC AGCCCCCTGCCAAAAGGTCGGAATCGGGGTCACCTTCTGCCGGAGGCCACAGCAAACCTCCTCACAGCCC ATTGGTCCTTAAGAGATGCCATGTGTCCACCCATCAGCACAATTATGCAGCGCCACCCTCCACCAGGAAG GACTATCCTGCTGCCAAGAGGGCTAAGTTGGACAATGGCAGGGTCCTGAAACAGATCAGCAACAACAGAA AATGTGCCAGCCCCAGGTCTTCGGACACGGAGGAGAATGACAAGAGGCGGACACACAACGTCTTGGAGC GCCAGAGGAGGAACGAGCTGAAACGCAGCTTTTTTGCCCTTCGTGACCAGATCCCGGAGTTGGAGAACAA TGAAAAAGCCCCCAAGGTAGTTATCCTTAAAAAAGCCACAGCCTACATCTTGTCCATCCAAGCAGAGGAGC AAAAGCTCATTTCAGAAAAAGACGTGTTGCGGAAGAGGCGAGAACAGTTGAAACTCAAACTTGAACAGCT

ACGGAACTCTTGTGCATAAATTGACCTATTGGAGGAAGGAACTGGAATCATTTGTGAATTCTCACTTGTT ACTAAGGGGGAATAAGGAAAAAGGTTTCTTCTGACAAAACTACAGCAACTCTTTACGAATGAACTTTTCT CAAATGCATGTTCAAGTGCAACCTCACAACCTTGGCTGGGTCTTGGGACTAATCCAGTCCCAAGGTTTAG CCATAATGTAAACTGCCTCAGATGGAACTTTGGGCATAAAAGAACTTTTTTTATGCTTGTCATCTTTTTT TTTTTTTCCCCTCTAACAGATTTGTATTTAAGAATTGTTTTTACAAAATCCTAAAGTTCACCCAATTTTC CTATGTAAATATGACCATTAAATGTAAATAACTTTAATAAAACGTTTATAGCAATTATACAAAATTTCAT GCCATGTATTATATAAAACATAATTTTTTTATTTAAGTACATTTTCCTTTTTAAAGTTGATTTTTTTTCT ATTGTTTTTAGAAAAAATAAAATACTTGGCAAATATATAATTGAGCCAAATCTT

>Camelusbactrianus(Bactriancamel)XM_010972639 GCTGTAGTAATTCCAGCGAGAGGCAGAGGGAGCGAGCGGGCGGGCGCTTGGAGGGTGGAAGAGCCGAG CCAGCAGAGCGAGCAGAGTCGCGCTCCGGGGACCCGGGGAAGGGGAGATCCGGAGTGAAAGGGGGGCT TCGCCTCCGGCCCGGCCGCCCCCAACCCACTCTTCCCGTCGCCCCCCGCCAGCAGTCCGCCACCCTCGCCAC ATCCACGAAACTTTGCCCACTGCGGCAGGCGGGCACTTTCCACTGGAACTTACAACACCGGAGCGACTCTC CCGACGCGGAGAGGCGATTCTGCCTATTTGGGGAGACGCTTTTCCCTGCCGCTGCCCACGACCCGCTCATCT GAAAGGCGCTCCACTCCGCCTTTTGGACGCTGGATTTCCTTCGGATAGTGGAAAACCCGCAGGCTGCCGCG ATGCCCCTCAATGTCAGCTTCACCAACAGGAACTATGACCTCGACTATGACTCGGTGCAGCCGTATTT CTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCGCCGGCGCCCAG CGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCCCGCCCCTGTCCCCAAGCCGCCGCTCCGGGCTCT GCTCTCCCTCCTACGTTGCGGTCGCGTCCTTCTCCCCCAGGGGAGACGACGACGGCGGCGGCGGCTTTTC CACTGCTGACCAGTTGGAGATGGTGACCGAGCTGCTGGGAGGAGATATGGTGAACCAGAGCTTCATATGC GACCCGGACGACGAGACCTTCATCAAAAATATCATCATCCAGGACTGTATGTGGAGCGGCTTCTCGGCCG CCGCCAAGCTCGTCTCCGAGAAGCTGGCCTCCTACCAGGCTGCGCGCAAAGACAGCAGCAGCCCGAGCCC CGCCCGTGGGCACGGCGGGTGCTCCCCCTCCAGCCTGTACCTGCAGGACCTAAGCGCCGCCGCCTCCGAA TGCATCGACCCCTCCGTGGTCTTCCCCTACCCGCTCAACGACAGCAGTTCGCCCAAGCCCTGCGCCTCCC CGGACTCCACCGCCTTCTCCCCGTCCTCGGACTCTCTGCTCTCCTCCGCCGAGTCGTCCCCGCGGGCCAG TCCCGAGCCCCTGGCGCTCCACGAGGAGACACCACCCACCACCAGCAGCGACTCTGAGGAAGAACAAGAG GATGAGGAAGAAATTGATGTTGTTTCTGTAGAGAAGAGGCAGCCCCCTGCCAAAAGGTCGGAATCGGGGT CACCTTCTGCCGGAGGCCACAGCAAACCTCCTCACAGCCCATTGGTCCTTAAGAGATGCCATGTGTCCAC CCATCAGCACAATTATGCAGCGCCACCCTCCACCAGGAAGGACTATCCTGCTGCCAAGAGGGCTAAGTTG GACAATGGCAGGGTCCTGAAACAGATCAGCAACAACAGAAAATGTGCCAGCCCCAGGTCTTCGGACACGG AGGAGAATGACAAGAGGCGGACACACAACGTCTTGGAGCGCCAGAGGAGGAACGAGCTGAAACGCAGC TTTTTTGCCCTTCGTGACCAGATCCCGGAGTTGGAGAACAATGAAAAAGCCCCCAAGGTAGTTATCCTTAAA AAAGCCACAGCCTACATCTTGTCCATCCAAGCAGAGGAGCAAAAGCTCATTTCAGAAAAAGACGTGTTGC GGAAGAGGCGAGAACAGTTGAAACTCAAACTTGAACAGCTACGGAACTCTTGTGCATAAATTGACCTATT GGAGGAAGGAACTGGAATCATTTGTGAATTCTCACTTGTTACTAAGGGGGAATAAGGAAAAAGGTTTCTT CTGACAAAACTACAGCAACTCTTTACGAATGAACTTTTCTCAAATGCATGTTCAAGTGCAACCTCACAAC CTTGGCTGGGTCTTGGGACTAATCCAGTCCCAAGGTTTAGCCATAATGTAAACTGCCTCAGATGGAACTT TGGGCATAAAAGAACTTTTTTTATGCTTGTCATCTTTTTTTTTTTTTCCCCTCTAACAGATTTGTATT TA AGAATTGTTTTTACAAAATCCTAAAGTTCACCCAATTTTCCTATGTAAATATGACCATTAAATGTAAATA ACTTTAATAAAACGTTTATAGCAATTATACAAAATTTCATGCCATGTATTATATAAAACATAATTTTTTT TATTTAAGTACATTTTCCTTTTTAAAGTTGATTTTTTTTCTATTGTTTTTAGAAAAAATAAAATACTTGG CAAATATATAATTGAGCCAAATCTT

>Microcebus murinus (gray mouselemur)XM_012785382 ACCACCCCCCCCCAACAAATTCAGTGGGAGTTTATTCGTTACGCGCTCTCCAAGTATACGTGGCAACGCG TTGCTGAGTTATTTTAATCATTCTAGGCATCATTTTCCTCCTTAGACCTCTATCATGTGCTCCGTCTACA CTGACACCCTGCATACTCAGCGCGCCCGGTAAAACCCTTCTTTCCTCCACTCTTCACGGGACTCGTTTTC AAAGCGCTGCCCTTTCCCCAGCCTTGGGGAGGCGCCCGGCCGCCTGGGTCGTGCGTGGCGCGGCCGTGG GTGCGCGGCGCCCTCGCAGTGTGCAGGCCAGCTGTTCCGCCTGCGACGACTGAAGCACGCAGGACGGGA ATGCGGTTCGCCACTCGGAGCGCTGCGCCAGAGACGCAGCAGAGAAAGGGAGAGGGCTCGAGCGAGAG CAAAAGAAAATGGCTGCCGCGACGATGCCCCTCAACGTCAGCTTCGCCAACAGAAACTATGACCTCGACTA CGACTCGGTGCAGCCGTATTTCTACTGCGATGAGGAGGAGAACTTCTACCAGCACCAGCAGCAGAGCGAGC TGCAGCCGCCGGCGCCCAGCGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCCCGCCCTTGTCCCCG AGCCGCCGCTCCTACGTTGCGGTCGCCGCGTCCTTCTCCCCCAGGGGAGACGACGACGGCGGCGGCGGCG GGAGCTTCTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGA GCTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGGCT TCTCGGCCGCCGCCAAGCTGGTGTCGGAGAAGCTGGCCTCCTACCAGGCTGCGCGCAAAGACAGCGGCAG CCCGAGCCCCGCCCGCGGGCCCAGCGGCTGCTCCACCTCCAGCTTGTACCTGCAGGACCTGAGCGCCGCC GCCTCCGAGTGCATCGACCCCTCCGTGGTCTTCCCCTACCCGCTCCATGACAGCAGCTCCCCCAAGCCCT GCGCCTCACCTGACTCCACCGCCTTCTCCTCGTCCTCGGACTCTCTGCTCTCCTCCACCGAGTCCTCCCC GCGGGCCAGCCCCGAGCCCCTGGCGCTTCACGAGGAAACACCACCCACCACCAGCAGCGACTCTGAGGAA GAACAGGAGGATGAGGAAGAAATCGATGTCGTCTCTGTGGAAAAGAGGCAGCCCCCTGCCAAAAGGTCA GAATCGGGGTCACCTTCTGGCGGAGGCCACAGCAGACCTCCTCACAGCCCGCTAGTCCTCAAGCGGTGCCA TGTCTCCACCCATCAGCACAATTACGCAGCGCCCCCCTCCACCAGGAAGGACTATCCCGCTGCCAAGAGG GCCAAGTTGGACAGTGTCAGAGTCCTGAGACAGATCAGCAACAACCGGAAATGCACCAGCCCCAGGTCCT CGGACACCGAGGAGAATGACAAGAGGCGGACACACAACGTCCTGGAGCGCCAGAGGAGGAACGAGCTG AAACGGAGCTTTTTTGCCCTGCGTGACCAGATCCCAGAGTTGGAAAACAACGAAAAAGCCCCCAAGGTAG TTATCCTTAAAAAAGCCACTGCATACATCCTGTCCATCCAAGCAGAGGAGCAAAAGCTCATTTCAGAAAAGG ACTCCTTGAGGAAGCGCCGAGAACAGTTGAAACACAAACTTGAACAGCTGCGGAACTCTTGTGCATAAGC GGACCTGTTGGAGGAAGGAACTGGAATGGCTCACGAATTCTCGCTTGTCACAAAGGGAAAGTAAGGAAAA AGGTTCCTTCCAACAGAACTGGCGCGATTTATTACCTATGAACCTATTTCAAATGCATGATCAGATGCAA CCTCACAACCTTGGCTGGGTCTTAGGACTGAAAGGCTTAGCCATAATGTAAACTGCCTCAAATGGGACTT TGGGCATAAAAGAACTTTTTTTATGCTTGCCATCTTTTTTTTGTTTTTTCCTTTAACAGATTTGTATTTA AGAATTGTTTTTAAAAGATTCTAAGGTTTACCCAATTTTTCTGTGTAAATATGGCCATTAAATGTAAATA ACTTTAATAAAACGTTTATAGCAGTTATACAAGATTTCAAACCATGTATTATAAAACCATAATTTTTTTA TTTAAGTACATTTTCCTTTTTAAAGTTGATTTTTTTTCTATTGTTTTTAGAAAAAATAAAATACCTGGCA AATATATAATTGAGCCAAA

>Ailuropoda melanoleuca (giant panda)XM_002914982 GCCGGCCGCGGGAAGGGCAGGGCTATTCAGAGCTTGGCGGGAAAAAGAGAACAGAGGGGGGCGGGATC GTGCCTAGCAATATAAAACCCGGTTTTCGGGCTTTATCCGACTCGCTGTAGTAATCCCAGCGAGAGACAGAG GGAGTGAGCGGGCGCGCCCGCTAGGGTGGAAGNNNNCGAGCGAGCCGAGCGAGCCGAGTGGCGCTCC GGGCGCCCGGGGAAGGGAGATCCGGAGCGAAAGGGGGCTTCGCCTCCGACCCAGCTGCCCCCACCCCAC CCGGACCGCCTACCCCTGCCAACGGGGCGCAACCCCCGGCGCCCCCACGAAACTTTGCCCGTTGCCGCGG GCGGACGCTTTCCCCTGGAACTTACAAGACCCGAGGGAGAGCGCGACTCTCCGGACGCGGAGAGGCTGTT CTGCCTATTTGGGGAGACACTTCTCCCCGCCGCTGCCCGCGACCGGCTCCTCTGAAAGGCGCTCCTCGCCG CTTTTTGGACGCTGGATTTCCTTCGGATAGTGGAAAACCCGGCTGCCGCGATGCCCCTGAACGTCAGCTTCG

CCAACAGGAACTATGACCTCGACTACGACTCGGTACAGCCTTATTTCTACTGCGACGAGGAGGAGAACTTC TACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCGCCGGCGCCCAGCGAGGATATCTGGAAGAAATTCGAGC TGCTGCCCACCCCGCCGCTGTCCCCGAGCCGCCGCTCGGGGCTCTGCTCGCCCTCCTACGTTGCAGTCGC GTCCTTCTCCCCCAGGGGGGACGACGACGGCGGCGGCGGCAGCTTTTCCACGGCCGACCAGTTGGAGATG GTGACCGAGCTGCTGGGAGGGGACATGGTGAACCAGAGCTTCATCTGCGACCCGGACGACGAGACCTTCA TCAAAAACATCATCATCCAGGACTGCATGTGGAGCGGCTTCTCGNGCGCCGCCAAGCTCGTCTCTGAGAA GCTGGCCTCCTACCAGGCTGCGCGCAAAGACAGCAGCAGCCCGAGCCCCGCCCGCGGGCCCGGCGGCTG CTCCACCTCCAGCCTGTACCTGCAGGACCTGAGCGCCGCCGCCTCCGAGTGCATCGACCCGTCCGTGGTCT TCCCCTACCCGCTCAACGACAGCAGCTCGCCCAAGCCCTGCGCCTCCCCGGACTCCACCGCCTTCTCCCC GTCCTCGGACTCTCTGCTCTCCTCGGCCGAGTCCTCCCCGCGGGCCAGCCCCGAGCCCCTGGCGCTCCAC GAGGAGACACCGCCCACCACCAGCAGCGACTCTGAGGAAGAACAAGAGGACGAAGAAGAAATTGATGTT GTTTCTGTGGAAAAAAGGCAGCCCCCTGCCAAAAGGTCAGAATCGGGGTCACCGTCGTCGGGAAGCCACA GCAAACCACCTCACAGCCCGCTGGTCCTTAAAAGATGCCATGTGTCCACCCATCAGCACAATTACGCAGCG CCCCCCTCCACCAGAAAGGACTATCCGGCCGCCAAGAGGGCTAAGTTGGACAGTGGCAGAGTCCTGAAAC AAATCAGCAACAACCGTAAATGTGCCAGCCCCAGGTCTTCGGACACAGAGGAGAACGACAAGAGGCGGAC ACACAACGTCTTAGAACGCCAGAGGAGAAACGAGCTGAAACGGAGCTTTTTTGCCCTGCGTGACCAGATC CCGGAGTTGGAAAACAATGAAAAGGCCCCCAAGGTTGTCATCCTTAAAAAAGCCACCGCGTACATCCTGT CCGTCCAAACAGAGGAGCAGAAGCTCATCTCAGAAAAGGACTTGTTGCGGAAGCGGCGAGAACAGTTGA AACACAAACTTGAACAGCTAAGGAACTCTGGTGCCTAAGTCCACCTATTGGAGGGAGGAACTGGAATTGGT CATGAATTCTCATTTGTTACTAAGAGAAAGTAAGGGAAAAGGTTCCTTTTGACAGAACTGTAGCAATTCC TCATATCTGAACTTTTTTCAAATGCATGTTCAAATGCAACCTCACAACCTAGGCTAGATCTTAGGATTGA AAGGTTTAGCCATGATGTAAACTGCCTCAAATAGAACTTTGGGCATAAAAGAACTTTTTTTTATGCTTGC CATCTTTTTTTTTTTCCTTTAACAGATTTGTATTTAAGAATTGTTTTTAAAAAATCGTAAAGTTTACCCC ATTTTCCCATGTAAATACGGTCATTAAATGTAAATAACTTTAATAAAACGTTTATAGCAGTTATACAAGA ATTCAAACCATGTATTATAAACCATAATTTTTTTTATTTAAGTACATTTTCCTTTTTAAAGTTGATTTTT TCTA

>Tupaia chinensis (Chinese tree shrew)XM_006156687 TTGAGGGGTCGCCCCTGCTCGCTCGGCCCCTTCGGGGGATAACACTGGAAGGGGAGTGGTTCGGGATCGT GGTGCGCATTAACGCCGGGCTTGGCATCACCAAGACGACCGTAACTTAACACTGTATGTGCCCTGTATGTGC CGCCTTCAGGCTGCCGCGACGATGCCCCTCAACGTCAGCTTCGCCAGCAGGAACTATGACCTCGACTACGA CTCGGTGCAGCCCTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTG CAGCCGCCCGCGCCCAGCGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCCCGCCCCTGTCAC CCAGCCGCCCATCCGGGCTCTGCTCGCCCTCCTACGTAGCCGTCGCGTCCTTCTCCCCCAGGGGAGACGA CGACGGCGGCGGCGGCAGTTTCTCCACCGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGA CATGGTGAACCAGAGCTTCATCTGCGACCCGGACGACGAGACCTTCATCAAGAACATCATCATCCAGGACT GTATGTGGAGCGGCTTCTCCGCCGCCGCCAAGCTGGTCTCCGAGAAGCTGGCCTCCTACCAGGCTGCGCG CAAAGACAGCGGCAGCCCGAGCCCCGCCCGCGGGCACAGCGGCTGCTCCACCTCCAGCTTGTACCTGCAG GACCTGAGCGCCGCCGCCTCCGAGTGCATCGACCCCTCGGTAGTCTTCCCCTACCCGCTCAACGACGGCA GCTCGCCCAAGCCCTGCGCCTCGCCGGACTCCACCGCCTTCTCCCCGTCCTCGGACTCCCTGCTCTCCTC CACCGAGTCCTCTCCGCGGGCCAGCCCCGAGCCCCTGGCGCTCCACGAGGAGACACCTCCCACCACCAGC AGCGACTCTGAGGAAGACCAAGAGGATGAGGAAGAAATTGATGTCGTTTCTGTAGAAAAGAGGCAGCCCC CTACCAAAAGGTCGGAATCGGGGTCACCCTCCGCTGGAGGCCACAGCAAACCCCCTCACAGTCCACTGGT TCTCAAGAGGTGCCATGTCTCCACCCATCAGCACAATTACGCCGCACCACCCTCCACCAGGAAGGACTAC

CCAGCTGCCAAGAGGGCTAAGCTGGACAGTGGCAGAGTCCTGAAACAGATCAGCAACAACCGGAAATGTG CCAGCCCCAGGTCCTCAGACACCGAGGAGAATGACAAGAGGCGGACACACAATGTCCTGGAACGCCAGA GGCGAAACGAGCTGAAACGAAGCTTTTTTGCTCTGCGTGACCAGATCCCCGAGTTGGAAAACAATGAAAA GGCCCCCAAAGTAGTTATCCTTAAAAAAGCCACAGCATACATCCTGTCTGTTCAAGCAGAGGAGCAAAAGC TCATTTCAGAAAAGGATTTGTTAAGGAAGCGGCGAGAACAGTTGAAACACAAACTTGAACAGCTACGGAA CTCTTGTGCATAAATTGACCTATTGGAGGGAGGGACTGGAGTCGCCTCTGAATTCTCACTTGTTACCAAG GGAAGTAAGGAAGAAGGTTCCTCCTTTCAGAACTTCTGGACTTGTTTTAAATGCATGGTCAAGTGCAACC GCACAACCTTGGCTGGGCCTTAGGACTGAAAGGTTTAGCCATAATGTAAAC

>Equus przewalskii (Przewalski's horse)XM_008544605 TAAAGGGGGCCTTCGCCTCCGGCCCAGCCGCCCCCACCCCACCCTTCCCGCCGACCCCTGCCAGCAGTGC GCAACCCCCGCCACATCCACGAAACTTTGCCCATTGCAGCGGGCGGGCACCCTCCACTGGAACTTACAAC ACCCGAGCGAGAACTCGACTCTCCGGACGCGGAGAGGCTATTCTGCCTATTTGGGGAGACACTTTTCCCC GCCACTGCCCACAACCCGCTCCTCTGAAAGGCGCTCCTCGCCGCTTTTCGGACGCTGGATTTCCTTCAGA TAGTGGAAAACCCGCAGGCTGCCGCGATGCCCCTCAACGTCAGCTTCGCCAGCAGGAACTATGACCTCGA CTACGACTCGGTGCAGCCCTATTTCTACTGCGACGAAGACGAGAACTTCTACCAGCAGCAGCAGCAGAGC GAGCTGCAGCCCCCGGCTCCCAGCGAGGATATCTGGAAGAAATTCGAGCTGCCCCCCCCGCCCCTGCCCC CGAGCCGCCGCTCCGGGTTCTGCTCGCCCTCCTATGTCGCGGTGGCGTCCTTCTCTCCCAGGGGAGACGA CGACGGCGGCGGCGGCAGCTTTTCCACGGCCGACCAGTTGGAGATGGTGACCGAGCTGCTGGGAGGAGA TATGGTGAACCAGAGCTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAACATCATCATCCAGGACT GTATGTGGAGGCCCGGCGGCTGCTCCACCTCCAGCTTGTACCTGCAGGACCTGAGCGCCGCCGCCTCCGA ATGCATCGACCCCTCCGTGGTCTTCCCCTACCCGCTCAACGACGGTAGCTCGCCCAAGCCCTGCGCCTCC CCCGACTCCACCGCCTTCTCTTCGTCCTCGGACTCTCTGCTCTCCTCGGCCGAGTCCTCCCCGCGGGCCA GCCCCGAGCCCCTGACGCTCCACGAGGAGACACCCCCCACCACCAGCAGCGACTCTGAAGAAGAACAAGA GGATGAGGAAGAAATTGATGTTGTTTCTGTGGAAAAGAGGCAGCCCCCTGCCAAAAGGTCAGAATCGGGG TCACCCTCTGCAGGAGGCCACAGCAAACCTCCTCACAGCCCCCTGGTCCTTAAGCGATGCCACGTGTCCA CCCACCAGCACAATTACGCAGCTCCCCCCTCCACTAGGAAGGACTATCCTGCCGCCAAGAGGGCTAAGTT GGACAGTGGCAGGGTCCTGAAACAGATCAGCAACAACCGGAAATGTACCAGCCCCAGGTCTTCGGACACA GAGGAGAATGACAAGAGGAGGACACACAACGTCCTGGAACGCCAGAGGAGAAATGAGCTGAAAAGGAG CTTTTTTGCCCTGCGAGACCAGATCCCTGAGTTGGAAAATAATGAAAAAGCTCCTAAAGTAGTTATCCTTAA AAAAGCCACCGTATTTATCTTGTCAGTCCAGGCAGAGGAGCAGAAGCTCGTTTCAGAAAAGGATTTACTG AGGAAGCGACGAGAACATTTGCAGCTTAAACTTGAACAGCTACGGAACTGTTGAGCATAGATTGACCTAT TGGAAGAAAGAACTGGAATTGCTCATGAATTCTCACTTGTTACTAAGAGAAAGTAAGGAAGAAAGTTCCT TAGGACAGAATTGCAGCAATTCCTGACTGAACTTTTTTCCAAATGCATGGTCAAATGTAACCTCACAACC TTGGCTGGGTCTTGGGACTGAAAGGTTTAGCCATAATGTAAACTGCCTCAAATGGAACTTAGAGCATAAA AGAACGTTTTTTATGCTTGCCATTTTTTTTTCCTTTAGCAGATTTGTATTTAAGAGTTGTTTTTAAAACA TGCTAAAATTTACCCCAATTTTCCTGTGTAAATATGGCCATTAAATGTAAATAATAAAACGTTTATAGCA GTTATGCAAAACTTCAAACCCTGTATTATAAACCATAATTTTTTTTATTTAAGTACATTTTCCTTTTTAA AGTTGATTTTTTTCTATTGTTTTTAGAAAAAAATAAAATACGTGGCAAATATATAATTGAGC

>Homo sapiens (human)k00534.1 GAGCAAAAGAAAATGGTAGGCGCGCGTAGTTAATTCATGCGGCTCTCTTACTCTGTTTACATCCTAGAGC TAGAGTGCTCGGCTGCCCGGCTGAGTCTCCTCCCCACCTTTCCCACCCTCCCCACCCTCCCCATAAGCGC CCCTCCCGGGTTCCCAAAGCAGAGGGCGTGGGGGAAAAGAAAAAAGATCCTCTCTCGCTAATCTCCGCCC

ACCGGCCCTTTATAATGCGAGGGTCTGGACGGCTGAGGACCCCCGAGCTGTGCTGCTCGCGGCCGCCACC GCCGGGCCCCGGCCGTCCCTGGCTCCCCTCCTGCCTCGAGAAGGGCAGGGCTTCTCAGAGGCTTGGCGGG AAAAAGAACGGAGGGAGGGATCGCGCTGAGTATAAAAGCCGGTTTTCGGGGCTTTATCTAACTCGCTGTA GTAATTCCAGCGAGAGGCAGAGGGAGCGAGCGGGCGGCCGGCTAGGGTGGAAGAGCCGGGCGAGCAGA GCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGGCTTCCGTCTGGCCCAGTCCTCC CGCTGATCCCCCAGTTAGCGGTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGGGCGGGCACTTT GCACTGGAACTTACAACACCCGAACACCCGAACACCCGAGCAAGGACGCGACTCTCCGACGCGGGGAGG CTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAG CTGCCTAGAGGATCCCCCTGAATCAGGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTTTTATCA CTTTAATGCTGAGATGAGTCGAATGCCTAAATAGGGTGTCTTTTCTCCCATTCCTGCGCTATTGACACTT CCCAGCCGCTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC CAGGTTTCCGCACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCT CCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGC CGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGC GCCCAGCGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCCCGCCCCTGTCCCCTAGCCGCCGCTCC GGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCG GGAGCTTCTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGA GTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGGC TTCTCGGCCGCCGCCAAGCTCGTCTCAGAGAAGCTGGCCTCCTACCAGGCTGCGCGCAAAGACAGCGGCA GCCCGAACCCCGCCCGCGGCCACAGCGTCTGCTCCACCTCCAGCTTGTACCTGCAGGATCTGAGCGCCGC CGCCTCAGAGTGCATCGACCCCTCGGTGGTCTTCCCCTACCCTCTCAACGACAGCAGCTCGCCCAAGTCC TGCGCCTCGCAAGACTCCAGCGCCTTCTCTCCGTCCTCGGATTCTCTGCTCTCCTCGACGGAGTCCTCCC CGCAGGGCAGCCCCGAGCCCCTGGTGCTCCATGAGGAGACACCGCCCACCACCAGCAGCGACTCTGGTAA GCGAAGCCCGCCCAGGCCTGTCAAAAGTGGGCGGCTGGATACCTTTCCCATTTTCATTGGCAGCTTATTT AACGGGCCACTCTTATTAGGAAGGAGAGATAGCAGATCTGGAGAGATTTGGGAGCTCAAGTGCTGGGATT ACAGGTGTGAGCCAGGGCACCAGGCTTAGATGTGGCTCTTTGGGGAGATAATTTTGTCCAGAGACCTTTC TAACGTATTCATGCCTTGTATTTGTACAGCATTAATCTGGTAATTGATTATTTTAATGTAACCTTGCTAA AGGAGTGATTTCTATTTCCTTTCTTAAAGAGGAGGAACAAGAAGATGAGGAAGAAATCGATGTTGTTTCT GTGGAAAAGAGGCAGGCTCCTGGCAAAAGGTCAGAGTCTGGATCACCTTCTGCTGGAGGCCACAGCAAAC CTCCTCACAGCCCACTGGTCCTCAAGAGGTGCCACGTCTCCACACATCAGCACAACTACGCAGCGCCTCC CTCCACTCGGAAGGACTATCCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGATC AGCAACAACCGAAAATGCACCAGCCCCAGGTCCTCGGACACCGAGGAGAATGTCAAGAGGCGAACACACA ACGTCTTGGAGCGCCAGAGGAGGAACGAGCTAAAACGGAGCTTTTTTGCCCTGCGTGACCAGATCCCGGA GTTGGAAAACAATGAAAAGGCCCCCAAGGTAGTTATCCTTAAAAAAGCCACAGCATACATCCTGTCCGTC CAAGCAGAGGAGCAAAAGCTCATTTCTGAAGAGGACTTGTTGCGGAAACGACGAGAACAGTTGAAACAC AAACTTGAACAGCTACGGAACTCTTGTGCGTAA

> Panthera tigris altaica (Amur tiger)XM_007076848 AATCTGGTCCTTGATTGCTGTGAGGACACGTCGCTAACCCGCGCGTTTTCTTTTTCCTTTCTTAAAGAGG AAGAACAAGAGGAAGAAGAAGAAATTGATGTCGTTTCTGTGGAGAAAAGGCAGCCCCCTGCCAAAAGGT CGGAATCGGGGTCACCCTCTGCCGGAGGTCACAGCAAACCTCCTCACAGCCCGCTGGTCCTTAAGAGATGC CACGTGCCCACCCATCAGCACAATTACGCAGCGCCCCCCTCCACTAGGAAGGACTACCCAGCCGCCAAGA GGGCTAAGTTGGACAGTGGCAGAGTCCTGAAACAGATCAGCAACAACCGCAAATGTATCAGCCCCAGGTC TTCGGACACGGAGGAGAACGACAAGAGGCGGACGCACAACGTCTTGGAACGCCAGAGGAGAAACGAGC