文心兰ACC氧化酶基因OnACO1克隆与表达分析

龙源期刊网 https://www.sodocs.net/doc/c618387795.html,

文心兰ACC氧化酶基因OnACO1克隆与表达分析

作者：杨光华刘进平

来源：《热带作物学报》2014年第04期

摘要以文心兰切花品种“黄金2号”（Oncidium Gower Ramsey ‘Gold2’）为材料，通过RT-PCR和RACE技术，从文心兰的花中分离得到了1个ACO基因成员的cDNA，命名为OnACO1（GeneBank登录号为JQ822088）。

中图分类号 Q75 文献标识码 A

Cloning and Expression Analysis of OnACO1 Gene in Oncidium

YANG Guanghua1，2，LIU Jinping1*

1 Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources，

Hainan University， Ministry of Education/College of Agronomy， Hainan University，Haikou， Hainan 570228，China

2 Sanya Science &Technology Academy for Crop Winter Multiplication， Sanya， Hainan 572000，China

Abstract A member of ACO gene family， OnACO1（GeneBank accession number

JQ822088） was cloned from Oncidium Gow er Ramsey ‘Gold2‘ by RT-PCR and RACE technology. The full length cDNA is 1 424 bp with an ORF of 972 bp encoding 324 amino acids，a 172 bp 5′-UTR and a 280 bp 3′-UTR. The deduced amino acid sequence of OnACO1 shares high homology with those of other orchid species. Vector pGEX-OnACO1 was constructed and transformed into E. coli BL21. Prokaryotic expression and SDS-PAGE indicated that a specific band of OnACO1 expression was in consistent with the predicted protein size. Quantitative PCR analysis showed there was a tissue-specific pattern of OnACO1 expression， mainly in gynostemium， and the expression was upregulated by ethylene.

Key words Oncidium；OnACO1；RACE；Gene cloning；Expression analysis

doi 10.3969/j.issn.1000-2561.2014.04.014

文心兰（Oncidium）又名舞女兰、跳舞兰、金蝶兰、瘤瓣兰，因其花色鲜黄，花型别致，花姿优美，是世界上重要的切花品种，具有较高的观赏价值。栽培的文心兰切花品种以黄色、